袁必锋

博士 , 教授
研究方向: 生物分析化学 / 化学生物学 / 色谱质谱分析
联系电话: 化西109B
Email: bfyuan@whu.edu.cn

教育与研究经历

1997.9-2001.6          武汉大学生命科学学院 生物化学专业        学士

2001.9-2006.6          武汉大学生命科学学院 生物物理专业        博士

2006.11-2007.6        新加坡国立大学生物系                             博士后

2007.7-2010.12        美国加州大学河滨分校化学系                   博士后

2011.1 至今              武汉大学化学与分子科学学院                   教授/博导

 

袁必锋: 博士,武汉大学化学与分子科学学院教授、博士生导师,国家自然科学基金优秀青年基金获得者(2015年)。2001年和2006年于武汉大学生命科学学院分别获得学士和博士学位。2006年至2010年在新加坡国立大学和加州大学河滨分校开展博士后研究。2011年任武汉大学化学与分子科学学院教授、博士生导师。担任Chinese Chemical Letters 副主编、Chemical Research in Toxicology 顾问编委 (Editorial Advisory Board)、Scientific Reports 编委、《色谱》编委、《分析测试学报》青年编委、《高等学校化学学报》青年编委、中国生物物理学会代谢组学分会理事。主要研究方向为生物分析化学、核酸化学生物学。在所从事的研究工作中,建立了DNARNA、蛋白质等多种生物大分子修饰(表观遗传修饰)的高灵敏分析方法,开展了生物大分子修饰和疾病的相关性研究;建立了多种代谢组学分析方法,开展了基于代谢组的临床疾病标志物分析。在PNASNucleic Acids ResearchChemical Science 等学术刊物上发表了190余篇SCI收录论文,专著一部,论文引用七千余次。


研究领域与兴趣

1. 核酸表观遗传修饰

核酸表观遗传修饰不仅在基因调控等方面有重要作用,而且在癌症等许多疾病的发生中也扮演着重要角色。本课题组通过建立质谱分析和测序等研究方法,对核酸表观遗传修饰的生理功能进行研究。

2. 代谢组学

发展基于质谱技术的代谢组学研究方法,结合相应的生物学手段,探索代谢物在疾病发生、发展过程中的生理功能。

3. 核酸损伤和修复

发展核酸损伤的检测手段,探索核酸损伤的定位、复制、转录、修复机制。  

 

招生专业:分析化学、化学生物学。课题组致力于化学和生物学交叉领域的研究工作,欢迎具有化学或生物学背景的学生、博士后加入。

 

项目

国家自然科学基金面上项目,22074110,基于质谱技术的DNA去甲基化新机制研究,2021/01-2024/12,主持。

国家自然科学基金创新研究群体,21721005,核酸识别和调控的化学生物学研究,2018/01-2023/12,参加。

国家重点研发计划精准医学研究重点专项,2017YFC0906800,临床样本代谢组的超灵敏高覆盖定量分析技术研究,2017/07-2019/12,参加。

国家自然科学基金面上项目,21672166,质谱探针技术研究重金属诱导的细胞表观遗传修饰动态变化及其机制,2017/01-2020/12,主持。

国家自然科学基金优秀青年基金,21522507,基于质谱技术的核酸修饰研究,2016/01-2018/12,主持。

 

课程

本科生课程:《化学生物学》

研究生课程:《生物分析化学》

 

学术成果

  1. Ma, C.J., Li, L., Shao, W.X., Ding, J.H., Cai, X., Lun, Z., Yuan, B.F.* and Feng, Y.Q. (2021) An enzyme-mediated bioorthogonal labeling method for genome-wide mapping of 5-hydroxymethyluracil. Chem Sci, 12, 14126-14132.

  2. Feng, Y., Chen, J.J., Xie, N.B., Ding, J.H., You, X.J., Tao, W.B., Zhang, X., Yi, C., Zhou, X., Yuan, B.F.* and Feng, Y.Q. (2021) Direct decarboxylation of Ten-eleven translocation-produced 5-carboxylcytosine in mammalian genomes forms a new mechanism for active DNA demethylation. Chem Sci, 12, 11322-11329.

  3. Cheng, M.Y., You, X.J., Ding, J.H., Dai, Y., Chen, M.Y., Yuan, B.F.* and Feng, Y.Q. (2021) Novel dual methylation of cytidines in RNA of mammals. Chem Sci, 12, 8149–8156.

  4. Dai, Y., Yuan, B.F.* and Feng, Y.Q. (2021) Quantification and mapping of DNA modifications. RSC Chem Biol, 2, 1096–1114.

  5. Dai, Y., Qi, C.B., Feng, Y., Cheng, Q.Y., Liu, F.L., Cheng, M.Y., Yuan, B.F.* and Feng, Y.Q. (2021) Sensitive and Simultaneous Determination of Uridine Thiolation and Hydroxylation Modifications in Eukaryotic RNA by Derivatization Coupled with Mass Spectrometry Analysis. Anal Chem. 93, 6938-6946.

  6. Liu, F.L., Ye, T.T., Ding, J.H., Yin, X.M., Yang, X.K., Huang, W.H., Yuan, B.F.* and Feng, Y.Q. (2021) Chemical Tagging Assisted Mass Spectrometry Analysis Enables Sensitive Determination of Phosphorylated Compounds in a Single Cell. Anal Chem, 93, 6848-6856.

  7. Feng, Y., Xie, N.B., Tao, W.B., Ding, J.H., You, X.J., Ma, C.J., Zhang, X., Yi, C., Zhou, X., Yuan, B.F.* and Feng, Y.Q. (2020) Transformation of 5-Carboxylcytosine to Cytosine Through C–C Bond Cleavage in Human Cells Constitutes a Novel Pathway for DNA Demethylation. CCS Chem, 2, 994-1008.

  8. Cheng, Q.Y., Xiong, J., Ma, C.J., Dai, Y., Ding, J.H., Liu, F.L., Yuan, B.F.* and Feng, Y. Q. (2020) Chemical tagging for sensitive determination of uridine modifications in RNA, Chem Sci, 11, 1878-1891.

  9. Ding, J.H., Ma, C.J., Chen, M.Y., Chen, B., Yuan, B.F.* and Feng, Y.Q. (2020) Quantification and single-base resolution analysis of N1-methyladenosine in mRNA by ligation-assisted differentiation. Anal Chem, 92, 2612-2619.

  10. Liu, F.L, Qi, C.B., Cheng, Q.Y., Ding, J.H., Yuan, B.F.* and Feng, Y.Q. (2020) Diazo reagent labeling with mass spectrometry analysis for sensitive determination of ribonucleotides in living organisms. Anal Chem, 92, 2301-2309.

  11. You, X.J., Liu, T., Ma, C.J., Qi, C.B., Tong, Y., Zhao, X., Yuan, B.F.* and Feng, Y.Q. (2019) Determination of RNA hydroxylmethylation in mammals by mass spectrometry analysis. Anal Chem, 91, 10477-10483.

  12. Tang, F., Liu, S., Li, Q.Y., Yuan, J., Li, L., Wang, Y., Yuan, B.F.* and Feng, Y.Q. (2019) Location analysis of 8-oxo-7,8-dihydroguanine in DNA by polymerase-mediated differential coding. Chem Sci, 10, 4272–4281.

  13. Xiong, J., Ye, T.T., Ma, C.J., Cheng, Q.Y., Yuan, B.F.* and Feng, Y.Q. (2019) N6-Hydroxymethyladenine: a hydroxylation derivative of N6-methyladenine in genomic DNA of mammals. Nucleic Acids Res, 47, 1268-1277.

  14. Chen, B., Yuan, B.F.* and Feng, Y.Q. (2019) Analytical Methods for Deciphering RNA Modifications. Anal Chem, 91, 743-756.

  15. Xu, J., Zhang, Q.F., Zheng, J., Yuan, B.F.* and Feng, Y.Q.* (2019) Mass spectrometry-based fecal metabolome analysis. TrAC-Trend Anal Chem, 112, 161-174.

  16. Ma, C.J., Ding, J.H., Ye, T.T., Yuan, B.F.* and Feng, Y.Q. (2019) AlkB Homologue 1 Demethylates N(3)-Methylcytidine in mRNA of Mammals. ACS Chem Biol, 14, 1418-1425.

  17. Li, Q.Y., Xie, N.B., Xiong, J., Yuan, B.F.* and Feng, Y.Q. (2018) Single-Nucleotide Resolution Analysis of 5-Hydroxymethylcytosine in DNA by Enzyme-Mediated Deamination in Combination with Sequencing. Anal Chem, 90, 14622-14628.

  18. Chu, J.M., Ye, T.T., Ma, C.J., Lan, M.D., Liu, T., Yuan, B.F.* and Feng, Y.Q. (2018) Existence of Internal N7-Methylguanosine Modification in mRNA Determined by Differential Enzyme Treatment Coupled with Mass Spectrometry Analysis. ACS Chem Biol. 13, 3243−3250

  19. Jiang, H.P., Xiong, J., Liu, F.L., Ma, C.J., Tang, X.L., Yuan, B.F.* and Feng, Y.Q. (2018) Modified nucleoside triphosphates exist in mammals. Chem Sci, 9, 4160-4167.

  20. Yuan, B.F., Zhu, Q.F., Guo, N., Zheng, S.J., Wang, Y.L., Wang, J., Xu, J., Liu, S.J., He, K., Hu, T., Zheng, Y.W., Xu, F.Q. and Feng, Y.Q.* (2018) Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis. Anal Chem, 90, 3512-3520.

  21. Lan, M.D., Xiong, J., You, X.J., Weng, X.C., Zhou, X., Yuan, B.F.* and Feng, Y.Q. (2018) Existence of Diverse Modifications in Small-RNA Species Composed of 16-28 Nucleotides. Chem-Eur J, 24, 9949-9956.

  22. Zeng, H., Qi, C.B., Liu, T., Xiao, H.M., Cheng, Q.Y., Jiang, H.P., Yuan, B.F.* and Feng, Y.Q. (2017) Formation and Determination of Endogenous Methylated Nucleotides in Mammals by Chemical Labeling Coupled with Mass Spectrometry Analysis. Anal Chem, 89, 4153-4160.

  23. Xiong, J., Liu, X., Cheng, Q.Y., Xiao, S., Xia, L.X., Yuan, B.F.* and Feng, Y.Q. (2017) Heavy Metals Induce Decline of Derivatives of 5-Methycytosine in Both DNA and RNA of Stem Cells. ACS Chem Biol, 12, 1636-1643.

  24. Tang, F., Yu, Q.W., Yuan, B.F.* and Feng, Y.Q.* (2017) Hydrophilic materials in sample pretreatment. TrAC-Trend Anal Chem, 86, 172-184.

  25. Huang, W., Lan, M.D., Qi, C.B., Zheng, S.J., Wei, S.Z., Yuan, B.F.* and Feng, Y.Q. (2016). Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 2016, 7, 5495-5502.

  26. Zhang, H.Y., Xiong, J., Qi, B.L., Feng, Y.Q.* and Yuan, B.F.* (2016) The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals. Chem Commun (Camb), 52, 737-740

  27. Huang, W., Qi, C.B., Lv, S.W., Xie, M., Feng, Y.Q.*, Huang, W.H.* and Yuan, B.F.* (2016) Determination of DNA and RNA Methylation in Circulating Tumor Cells by Mass Spectrometry. Anal Chem. 88, 1378-1384.

  28. Xu, J., Wu, P., Ye, E.C., Yuan, B.F.* and Feng, Y.Q.* (2016) Metal oxides in sample pretreatment. TrAC-Trend Anal Chem, 80, 41-56.

  29. Li, X.S., Yuan, B.F.* and Feng, Y.Q.* (2016) Recent advances in phosphopeptide enrichment: Strategies and techniques. TrAC-Trend Anal Chem, 78, 70-83.

  30. Tang, Y., Zheng, S.J., Qi, C.B., Feng, Y.Q.* and Yuan, B.F.* (2015) Sensitive and simultaneous determination of 5-methylcytosine and its oxidation products in genomic DNA by chemical derivatization coupled with liquid chromatography-tandem mass spectrometry analysis. Anal Chem, 87, 3445-3452.

  31. Chu, J.M., Qi, C.B., Huang, Y.Q., Jiang, H.P., Hao, Y.H., Yuan, B.F.* and Feng, Y.Q.* (2015) Metal Oxide-Based Selective Enrichment Combined with Stable Isotope Labeling-Mass Spectrometry Analysis for Profiling of Ribose Conjugates. Anal Chem, 87, 7364-7372.

  32. Shen, F., Huang, W., Huang, J.T., Xiong, J., Yang, Y., Wu, K., Jia, G.F., Chen, J., Feng, Y.Q.*, Yuan, B.F.*, Liu, S.M.* (2015) Decreased N(6)-Methyladenosine in Peripheral Blood RNA From Diabetic Patients Is Associated With FTO Expression Rather Than ALKBH5. J Clin Endocrinol Metab, 100, E148-154.

  33. Yuan, B.F.* and Feng, Y.Q. (2014) Recent advances in the analysis of 5-methylcytosine and its oxidation products. TrAC-Trend Anal Chem, 54, 24-35.

  34. Xing, X.W., Tang, F., Wu, J., Chu, J.M., Feng, Y.Q., Zhou, X.* and Yuan, B.F.* (2014) Sensitive detection of DNA methyltransferase activity based on exonuclease-mediated target recycling. Anal Chem, 86, 11269-11274.

  35. Tang, Y., Xiong, J., Jiang, H.P., Zheng, S.J., Feng, Y.Q.* and Yuan, B.F.* (2014) Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis. Anal Chem, 86, 7764-7772.

  36. Qi, B.L., Liu, P., Wang, Q.Y., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Derivatization for liquid chromatography-mass spectrometry. TrAC-Trend Anal Chem, 59, 121-132.

  37. Liu, P., Huang, Y.Q., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Profiling of thiol-containing compounds by stable isotope labeling double precursor ion scan mass spectrometry. Anal Chem, 86, 9765-9773.

  38. Wang, S.T., Huang, W., Lu, W., Yuan, B.F.* and Feng, Y.Q.* (2013) TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleosides before Detection of 2'-Deoxynucleosides/Low-Abundance 2'-O-Modified Ribonucleosides. Anal Chem, 85, 10512-10518.

  39. Tang, Y., Chu, J.M., Huang, W., Xiong, J., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Hydrophilic material for the selective enrichment of 5-hydroxymethylcytosine and its liquid chromatography-tandem mass spectrometry detection. Anal Chem, 85, 6129-6135.

  40. Chen, M.L., Shen, F., Huang, W., Qi, J.H., Wang, Y., Feng, Y.Q.*, Liu, S.M.* and Yuan, B.F.* (2013) Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry. Clin Chem, 59, 824-832.

  41. Chen, M.L., Liu, Y.L., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA Methylation. Chem-Eur J, 19, 1035-1041.

  42. Tang, Y., Gao, X.D., Wang, Y., Yuan, B.F.* and Feng, Y.Q.* (2012) Widespread existence of Cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal Chem, 84, 7249-7255.

  43. Yuan, B.F., Zhang, J., Wang, H., Xiong, L., Cai, Q., Wang, T., Jacobsen, S., Pradhan, S. and Wang, Y.* (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. Cancer Res, 71, 1904-1911.

  44. Yuan, B.F., Wang, J., Cao, H., Sun, R. and Wang, Y.* (2011) High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing. Nucleic Acids Res, 39, 5945-5954.

  45. Yuan, B.F., O'Connor, T.R. and Wang, Y.* (2010) 6-Thioguanine and S(6)-Methylthioguanine Are Mutagenic in Human Cells. ACS Chem. Biol., 5, 1021-1027.

  46. Yuan, B.F., Cao, H., Jiang, Y., Hong, H. and Wang, Y.* (2008) Efficient and accurate bypass of N2-(1-carboxyethyl)-2'-deoxyguanosine by DinB DNA polymerase in vitro and in vivo. Proc. Natl. Acad. Sci. U S A., 105, 8679-8684.

  47. Yuan, B.F., Zhuang, X.Y., Hao, Y.H. and Tan, Z.* (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb), 6600-6602.

  48. Yuan, B.F., Hao, Y.H. and Tan, Z.* (2004) Universal sensing strategy for the detection of nucleic acid targets by optical biosensor based on surface plasmon resonance. Clin Chem, 50, 1057-1060.


发表SCI论文190余篇,论文列表:

http://scholar.google.com/citations?hl=enundefinedamp;user=jrzBHhAAAAAJundefinedamp;view_op=list_worksundefinedamp;pagesize=100


http://orcid.org/0000-0001-5223-4659


实验室网站

http://bioanalchem.whu.edu.cn/index.html